Selection of cell culture plates

The cell culture plate can be divided into flat bottom and round bottom (U-shaped and V-shaped) according to the shape of the bottom; the number of culture holes is 6, 12, 24, 48, 96, 384, 1536, etc .; depending on the material Terasaki plates and ordinary cell culture plates. The specific selection depends on the type of cultured cells, the required culture volume and different experimental purposes.

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    The cell culture plate can be divided into flat bottom and round bottom (U-shaped and V-shaped) according to the shape of the bottom; the number of culture holes is 6, 12, 24, 48, 96, 384, 1536, etc .; depending on the material Terasaki plates and ordinary cell culture plates. The specific selection depends on the type of cultured cells, the required culture volume and different experimental purposes.


(1) Difference and selection of flat-bottom and round-bottom (U-shaped and V-shaped) culture plates


    Different shapes of culture plates have different uses. Cultured cells are usually selected with flat bottoms, which is convenient for observation under a microscope, has a clear bottom area, and the cell culture liquid level is relatively consistent. Therefore, when doing experiments such as MTT, whether it is adherent or suspended cells, generally use a flat bottom plate. For measuring absorbance, a flat-bottom culture plate must be used. Pay special attention to the material. The label "Tissue Culture (TC) Treated" is used for cultured cells.


    U-shaped or V-shaped boards are generally used only when there are special requirements. For example, in the field of immunology, when two different lymphocytes are mixed and cultured, they need to be in contact with each other to stimulate. In this case, U-shaped plates are generally selected because the cells will gather in a small range due to the effect of gravity. The round-bottom culture plate will also be used for the experiment of isotope incorporation, and it is necessary to use a cell collector to collect the cell culture, such as "mixed lymphocyte culture". V-shaped plates are commonly used for cell killing and immunological hemagglutination experiments. Cell killing can also be replaced by U-shaped plates (after adding cells, centrifuge at low speed).

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(2) The difference between Terasaki plate and ordinary cell culture plate


     Terasaki plate is mainly used for crystallography research, and the product design is convenient for observation and structure analysis of crystals. There are two methods of sitting and handing drop, and the appearance and structure of the products applied in the two methods are also different. Choose crystal class polymer as the material. Special materials are helpful for observing the crystal structure.


     The cell culture plate is mainly made of PS material. The material is treated sufface, which is convenient for the growth and extension of the cells. Of course, there are growth materials for planktonic cells, as well as low binding surface

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(3) Difference between cell culture plate and enzyme plate


     The enzyme plate is generally more expensive than the cell culture plate. The cell plate is mainly used for cell culture, and can also be used to measure protein concentration; the enzyme plate includes a coating plate and a reaction plate, which is generally not used for cell culture. After protein detection, higher requirements and specific enzyme-labeled working solutions are required.

(4) The well bottom area of commonly used different culture plates and the recommended amount of liquid added


     The liquid surface of the culture medium added in different well plates should not be too deep, generally in the range of 2 ~ 3mm, combined with the bottom area of different wells to calculate the appropriate amount of liquid for each culture well (refer to the following table). If the amount of liquid added is too large, it will affect the exchange of gas (oxygen), and it is easy to overflow and cause pollution during the moving process. The specific cell density to be added is flexibly controlled according to the purpose of the experiment.



培养器皿

底面积(cm2)

加培养液量(mL)

可获细胞量

96孔培养板

0.32

0.1

105

24孔培养板

2

1.0

5×105

12孔培养板

4.5

2.0

106

6孔培养板

9.6

2.5

2.5×106

4孔培养板

28

5.0

7×106

3.5cm培养皿

8

3.0

2.0×106

6cm培养皿

21

5.0

5.2×106

9cm培养皿

49

10.0

12.2×106

10cm培养皿

55

10.0

13.7×106

25cm塑料培养瓶

25

5.0

5×106

75cm塑料培养瓶

75

15~30

2×107

25cm玻璃培养瓶

19

4.0

3×106

100cm玻璃培养瓶

37.5

10.0

6×106

250cm玻璃培养瓶

78

15.0

2×107

2500cm旋转培养瓶

700

100~250

2.5×108

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